Methods of detection employing immuno-Q-Amp technology
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United States of America Patent
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app pub date -
Sep 28, 2005
filing date -
Nov 10, 2003
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Abstract
Disclosed herein are methods of detecting target molecules from a complex sample using a modified affinity ligand. In particular, the modified affinity ligand is an antibody specific for the target and has conjugated to it a nucleic acid molecule. This conjugated nucleic acid molecule serves as a template for a replicase that can replicate the template a billion-fold in an matter of minutes. The replication products then serve to facilitate a detectable signal indicating the presence of the target in the sample.
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