Recovery of original template
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United States of America Patent
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Issued Date -
Feb 16, 2006
app pub date -
Dec 2, 2003
filing date -
Dec 2, 2002
priority date (Note) -
Abandoned
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Abstract
The present invention relates to methods for regenerating a single-stranded nucleic acid template following its conversion to a double-stranded product, e.g., during a polymerase reaction, and also to regenerating a hairpin or anchoring sequence by removal of the template and its synthesized complement, by design of enzyme restriction sites into the hairpin or anchoring sequence.
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