Protein microarrays
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United States of America Patent
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Issued Date -
Feb 23, 2006
app pub date -
Aug 17, 2004
filing date -
Aug 17, 2004
priority date (Note) -
Abandoned
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Abstract
Methods for making a microarray having minimal background binding of proteins by appropriately coating a substrate surface which is initially derivatized with organic functional groups. A protein-resistant polymeric coating is applied which has hydrophilic backbone polymers that are crosslinked to a substantial degree via polyfunctional isocyanate moieties. Three dimensional hydrogel microspots containing capture agents are affixed at distinct spatial locations across an array region of the surface to form a microarray. The microspots are affixed either to the substrate or to the coating. The polymeric coating preferably comprises isocyanate-capped PEG crosslinked with a polyfunctional isocyanate to form urethane polymers.
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