Rapid generation of activated mononuclear antigen presenting cells from monocytes
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United States of America Patent
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Issued Date -
Apr 6, 2006
app pub date -
Apr 15, 2003
filing date -
Apr 19, 2002
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Abstract
A cell population prepared from blood mononuclear cells, the mononuclear cells being peripheral blood mononuclear cells (PBMC) or CD 14+ monocytes, the population containing from about 10% to about 50% of activated mononuclear antigen presenting cells (APCs) having the following characteristics: .cndot. they express surface markers CD2, CD83 and CD14, MHC class I and MHC class II molecules .cndot. they secrete TNF-.alpha. .cndot. they are able to stimulate allogenic T lymphocytes, as shown by MLR measurements .cndot. they are able to stimulate autologous T lymphocytes proliferation in an antigen specific way.
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