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Abstract

The present diagnostic test kit and methods of diagnosis and treatment of Helicobacter spp. associated infections provides accurate diagnosis and treatment for Helicobacter spp. related infections and diseases. The method of diagnosis includes analyzing a tissue specimen from a patient diagnosed with a disease. The tissue specimen can be a tumor cell related to the diagnosed disease of the patient. The tissue specimen is analyzed for the presence of Helicobacter spp. DNA or its components. One such analysis includes fluorescent in-situ hybridization (FISH). Once the presence of Helicobacter spp. DNA and/or its components is detected, the patient may be treated with a host of treatments including antibiotic therapy, molecular signal therapy, vaccination, interference with receptor systems mediating Helicobacter spp. invasion into tissues, and use of small-interfering RNA sequences to specifically block virulence or replication of Helicobacter spp. These treatments are administered for a duration to eradicate the Helicobacter spp. in the tissues of the patient. A test kit is also provided for quickly determining the presence of unspecified Helicobacter spp. DNA in a tissue specimen of a patient.

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