US Patent Application No: 2006/0085,871

Number of patents in Portfolio can not be more than 2000

Systems and methods for clonal expression in plants

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Abstract

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The present invention provides systems and methods for generating clonal root lines, clonal root cell lines, clonal plant cell lines, and clonal plants and for expressing gene products in such cell lines and plants. According to certain of the inventive methods, a viral vector containing a polynucleotide of interest operably linked to a promoter is introduced into a plant or portion thereof. Plant material (e.g., leaf portions) containing the viral vector are used to generate clonal root lines, e.g., by contacting them with A. rhizogenes so as to form hairy roots. The hairy roots are derived from single ancestral cells and are therefore clonal. These clonal root lines contain the viral vector and express the polynucleotide of interest. Clonal root cell lines, clonal plant cell lines, and clonal plants are obtained from the clonal roots. According to certain other inventive methods, a viral vector containing a polynucleotide of interest operably linked to a promoter is introduced into cells of a plant cell line (e.g., pre-existing or newly derived) that is maintained in culture. Clonal plant cell lines that express the polynucleotide of interest are obtained, e.g., by successive rounds of enrichment until a population of cells exhibiting stable expression is obtained. Clonal plants whose cells contain the viral vector are obtained from the clonal plant cell lines using standard methods. The invention provides clonal root lines, clonal root cell lines, clonal plant cell lines, and clonal plants generated using the inventive methods.

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First Claim

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Patent Owner(s)

Patent OwnerAddressTotal Patents
IBIO INC.NEWARK, DE26

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Skarjinskaia, Marina Newark, DE 6 31
Yusibov, Vidadi Havertown, PA 40 231

Cited Art Landscape

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* 2006/0085,871 Systems and methods for clonal expression in plants 7 2005
* 2006/0277,634 Production of foreign nucleic acids and polypeptides in sprout systems 6 2006
* 2008/0241,931 System For Expression of Genes In Plants 5 2008
* Cited By Examiner

Patent Citation Ranking

Forward Cite Landscape

Patent Info (Count) # Cites Year
 
ICON GENETICS GMBH (2)
* 8,940,963 Production of hetero-oligomeric proteins in plants 0 2006
* 2009/0111,145 PRODUCTION OF HETERO-OLIGOMERIC PROTEINS IN PLANTS 0 2006
 
iBio, Inc. and Fraunhofer USA, Inc. (1)
7,683,238 Production of pharmaceutically active proteins in sprouted seedlings 3 2002
 
iBio Inc. (10)
* 2004/0093,643 Production of pharmaceutically active proteins in sprouted seedlings 18 2002
* 2005/0114,920 Expression of foreign sequences in plants using transactivation system 14 2004
8,148,608 Systems and methods for clonal expression in plants 0 2005
* 2006/0085,871 Systems and methods for clonal expression in plants 7 2005
7,692,063 Production of foreign nucleic acids and polypeptides in sprout systems 3 2006
* 2006/0277,634 Production of foreign nucleic acids and polypeptides in sprout systems 6 2006
8,058,511 System for expression of genes in plants 3 2008
* 2011/0070,609 Production of Foreign Nucleic Acids and Polypeptides in Sprout Systems 2 2010
8,597,942 System for expression of genes in plants 2 2011
8,951,791 System for expression of genes in plants 0 2013
* Cited By Examiner