Primer and probe for detecting vibrio cholerae or vibrio mimicus and detection method using the same

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United States of America Patent

APP PUB NO 20060115820A1
SERIAL NO

10538636

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To produce high-performance specific gene amplification primers for detecting, quantifying, or identifying Vibrio cholerae and Vibrio mimicus, having low risk of misidentification and practically sufficient amplification efficiency and amplification specificity. We have determined partial nucleotide sequences of rpoD and gyrB genes of Vibrio cholerae, Vibrio mimicus, and closely related species, revealed their phylogenetic relationship, and then identified nucleotides characteristic of Vibrio cholerae and Vibrio mimicus, respectively. Thus, we have made it possible to design probes having high specificity and gene amplification primers having high specificity and excellent amplification efficiency, both of which contain the characteristic nucleotides.

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NICHIREI FOODS INC19-20 TSUKIJI 6-CHOME CHUO-KU TOKYO 1048402 ?1048402

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Fukuyama, Masafumi Kanangawa, JP 2 1
Furuhata, Katsunori Kanagawa, JP 2 1
Koizumi, Takeshi Chiba, JP 23 136
Nishiyama, Yoko Chiba, JP 6 24
Oonaka, Kenji Kanagawa, JP 2 1
Yamamoto, Satoshi Chiba, JP 514 4290

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