Apparatus and method of utilizing a sawed tooth shaped gradient for chromatographic separation
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United States of America Patent
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Issued Date -
Jun 8, 2006
app pub date -
Jun 15, 2005
filing date -
Jun 15, 2004
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The present invention provides an apparatus and a method wherein the movement of analytes on a chromatographic separation column is controlled by the manipulation of isocratic and gradient flow. Such manipulation allows for a distinct set of analytes to be eluted from the chromatography column and delivered downstream for further separation and/or processing while the remainder of the sample remains in a 'holding pattern' on the chromatography column. As such, the present invention allows for a small portion of the sample to be processed downstream of the column while substantially eliminating undesirable isocratic elution from the column during such downstream processing. Once the downstream processing has been completed, the column of the present invention elutes a second distinct analyte and the remainder of the sample is maintained in a holding pattern. The process may be repeated until the entire sample has been eluted from the chromatography column.
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