Cell culture method and cultured tissue
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United States of America Patent
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Issued Date -
Jun 8, 2006
app pub date -
Nov 14, 2005
filing date -
May 14, 2003
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
A cell culture method prepares first cells that are adhesion-dependent cells and monolayered or multilayered cells on a culture surface of a culture substrate, seeds second cells that are adhesion-dependent cells and are magnetized by allowing to have magnetic particles on the first cells, induces the second cells to a predetermined position on the first cells by magnetic force, and cultures the first cells and the second cells in a cell arrangement obtained by the magnetic induction. According to this cell culture method, after a cell sheet was prepared individually, cells can be multilayered without changing a temperature, peeling the monolayered sheet and laminating the monolayered sheets.
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