Recovery of virus from cell culture using a hypertonic salt solution
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United States of America Patent
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Issued Date -
Jun 29, 2006
app pub date -
Nov 29, 2005
filing date -
Aug 7, 1997
priority date (Note) -
Abandoned
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Abstract
A process of harvesting a herpesvirus from a cell culture infected therewith comprises treating said culture with a hypertonic aqueous salt solution to yield a virus suspension, e.g. to give improved yield of virus for live virus vaccine where otherwise cell-disruption might be used to harvest the virus by disrupting virus-infected cells. The harvesting step can be followed e.g. by nuclease treatment, diafiltration and lyophilisation.
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