Importance

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Abstract

An apparatus and method for liquid sampling utilizing a ribbed pipette tip for barrier penetration is disclosed herein. The unique, disposable plastic pipette tip of the present invention has an outer surface with at least three ribs extending longitudinally along the outer surface of the barrel. Each rib is circumferentially spaced from one another at a uniform distance, with each rib being symmetrically sized and positioned on the pipette tip barrel. The pipette tip is capable of being placed on a mounting shaft of a hand-held pipette or on the mounting head of an automated liquid handling machine. The pipette tip is rigid and straight enough to pierce a barrier sheet or resilient barrier sealing a container holding a liquid to be sampled. The ribs of the pipette tip operate to keep the barrier separated from the outer surface of the pipette tip such that ambient air is allowed to flow into and from the interior of the sealed container assembly during aspiration of the liquid sample into the pipette tip allowing for accurate transfer of liquids while minimizing the risk of contamination. The pipette tip and method of the present invention may be utilized in an automated pipetting system to sample an array of sealed containers, such as sealed wells and the pipette tip is sufficiently. The pipette of the present invention may include a self-sealing filter to prevent cross-contamination.

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First Claim