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Abstract

The present invention is in the field of nucleic acid-based diagnostic assays. More particularly, it relates to methods useful for the 'diagnostic sequencing' of regions of sample nucleic acids for which a prototypic or reference sequence is already available (also referred to as 're-sequencing'), or which may be determined using the methods described herein. This diagnostic technology is useful in areas that require such re-sequencing in a rapid and reliable way: (i) the identification of the various allelic sequences of a certain region/gene, (ii) the scoring of disease-associated mutations. (iii) the detection of somatic variations, (iv) studies in the field of molecular evolution, (v) the determination of the nucleic acid sequences of prokaryotic and eukaryotic genomes; (vi) identifying one or more nucleic acids in one or more biological samples; (vii) and determining the expression profile of genes in a biological sample and other areas.

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First Claim