Method for isolating intracellular antibodies able to neutralize protein interactions
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United States of America Patent
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Issued Date -
Nov 16, 2006
app pub date -
Nov 21, 2003
filing date -
Nov 21, 2002
priority date (Note) -
Abandoned
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Abstract
A method is described for isolating neutralizing intracellular antibodies of an interaction between a protein ligand x and a protein ligand y inside a cell. A method to identify a protein ligand x able to bind to a known y ligand using intracellular antibodies able to disrupt the interaction between x and y is also described. A method for the isolation of a set of antibody fragments against a significant proportion of the protein-protein interactions of a given cell (interactome) or against the protein interactions that constitute an intracellular pathway or network is also described.
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