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Abstract

A plating medium for identification of Enterobacter sakazakii bacteria having a carbohydrate, but Enterobacter sakazakii bacteria being incapable of fermenting any carbohydrate in the medium. The medium also contains a pH indicator dye that changes the color of the medium from a first color to a second color when the pH changes, first and second chromogenic substrates that react to alpha-glucosidase and beta cellobiosidase enzymes, respectively, to produce a third color in the medium, and agar to solidify the mixture. Microorganisms that ferment the carbohydrate but do not produce alpha-glucosidase or beta-cellobiosidase will produce colonies of the second color, microorganisms that produce alpha-glucosidase and/or beta-cellobiosidase including Enterobacter sakazakii bacteria will produce colonies of the third color, and microorganisms that ferment the carbohydrate and produce alpha glucosidase and/or beta-cellobiosidase will produce colonies of a fourth color which is the color that results from mixing the second and third colors.

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First Claim