High throughput multi-antigen microfluidic fluorescence immunoassays

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United States of America Patent

APP PUB NO 20060263818A1
SERIAL NO

11439288

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The development of a high-throughput multi-antigen microfluidic fluorescence immunoassay system is illustrated in a 100-chamber PDMS (polydimethylsiloxane) chip which performs up to 5 tests for each of 10 samples. Specificity of detection is demonstrated and calibration curves produced for C-Reactive Protein (CRP), Prostate Specific Antigen (PSA), ferritin, and Vascular Endothelial Growth Factor (VEGF). The measurements show sensitivity at and below levels that are significant in current clinical laboratory practice (with SIN>8 at as low as 10 pM antigen concentration). The chip uses 100 nL per sample for all four tests and provides an improved instrument for use in scientific research and 'point-of-care' testing in medicine.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Anderson, W French San Gabriel, CA 44 1416
Kartalov, Emil P Los Angeles, CA 37 212
Scherer, Axel Laguna Beach, CA 328 8232
Taylor, Clive South Pasadena, CA 11 123

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