Importance

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Abstract

Methods for quantitatively measuring the amount of one or more analyte(s) of interest in a fluid sample, and kits useful in the methods, are disclosed. The methods involve providing a solid phase apparatus comprising a membrane having an application point, a sample capture zone, and a control capture zone, where the sample capture zone and the control capture zone are approximately equidistant from the application point; and providing a sample collection apparatus comprising one or more population(s) of analyte binding particles. In the assays, a fluid sample is introduced into the sample collection apparatus, and the resultant mixture is applied to the application point of the membrane. The fluid allows transport components of the assay by capillary action to and through the sample capture zone(s) and the control capture zone. The amount of each analyte of interest in the fluid sample is related (e.g., either directly or inversely) to a corrected particle amount, which can be determined, for example, as a ratio of the amount of particles in the corresponding sample capture zone and the amount of particles in the control capture zone.

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First Claim