Parametric imaging of gene expression in biopsy samples utilizing the reverse transcriptase-polymerase chain reaction method
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United States of America Patent
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Issued Date -
Jun 21, 2007
app pub date -
Dec 15, 2006
filing date -
Dec 20, 2005
priority date (Note) -
Abandoned
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Abstract
A method is described for generating and showing the profile of one or more genes across a tissue sample. Tissue removed from the body is sliced in to thin sheets and those sheets are then divided into small portions each portion being identified as to a location in the sheet. The image of the thin slice and the position of each small portion thereof is recorded in a computer in a manner that can generate an image of the slice. Each small portion is subject to RT-PCR to identify the presence and quantity of one or more genes therein. The portion-specific data is then entered into the computer and an image of the slice is generated showing the gene specific characteristics of each small portion. The result is a parametric image of the entire slice which allows the visualization of the gene expression within each portion which can then be compared with other images of the same or adjacent tissue.
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