Method, reagent and kit for determination of cholesterol in very low-density lipoprotein remnant (vldl remnant)
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United States of America Patent
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Issued Date -
Jul 12, 2007
app pub date -
Jan 28, 2005
filing date -
Jan 29, 2004
priority date (Note) -
Abandoned
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Abstract
According to the present invention, esterified and free cholesterol in very low-density lipoprotein remnant (hereinafter collectively referred to as very low-density lipoprotein remnant cholesterol) in a sample is determined in an aqueous medium containing the sample in the presence of a combination of (a) a polyoxyethylene-polyoxyalkylene alkylaryl ether and (b) one kind of surfactant selected from the group consisting of a polyoxyethylene-polyoxybutylene copolymer, a polyoxyethylene styrenated-phenyl ether and a polyoxyalkylene long-chain alkyl ether which combination is capable of causing cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase to act specifically on very low-density lipoprotein remnant cholesterol.
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