Detection mycobacterium
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United States of America Patent
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Issued Date -
Jul 19, 2007
app pub date -
Aug 9, 2005
filing date -
Aug 9, 2004
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Abandoned
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Abstract
The present invention provides a method for determining the presence of Mycobacterium avium subspecies paratuberculosis (MAP) in a sample. The method involves using a pair of oligonucleotide probes and detecting the presence or absence of fluorescence resonance energy transfer (FRET) between the donor fluorescent moiety of the first probe and the corresponding acceptor fluorescent moiety of the second probe. The present invention also provides a method for isolating and/or extracting DNA from a microorganism.
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