Novel vector and method of constructing transformant plant using the vector
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United States of America Patent
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Issued Date -
Jul 19, 2007
app pub date -
Apr 7, 2004
filing date -
Apr 7, 2003
priority date (Note) -
Abandoned
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Abstract
Gene introduction to a plant is carried out by using a vector which comprises the following constitutional units A and B: A: a DNA sequence comprising P1, an expression inhibitory sequence and a desired gene which is controlled by P1 and the expression inhibitory sequence; B: a DNA sequence comprising P2, an expression inhibitory gene which is controlled by P2, P3, the expression inhibitory sequence, and a gene of a removal reaction-catalyzing enzyme which is controlled by P3 and the expression inhibitory sequence, wherein the DNA sequence is removed by expression of the gene of a removal reaction-catalyzing enzyme, wherein P1 is a promoter which shows its activity in at least a callus and a plant tissue where a desired gene should be expressed, P2 is a promoter which shows its activity in at least a callus, and P3 is a promoter which shows its activity in at least a plant tissue where P1 and P2 do not show their activities.
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