DNA diagnostics based on mass spectrometry
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United States of America Patent
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Mar 10, 2009
Grant Date -
Aug 30, 2007
app pub date -
Oct 2, 2006
filing date -
Nov 6, 1996
priority date (Note) -
Expired
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Abstract
Provided are methods for detecting a target nucleic acid in a biological sample using RNA amplification. In an embodiment, provided is a method that comprises (a) amplifying a target nucleic acid, or portion thereof, using a primer comprising a sequence that is complementary to a polynucleotide sequence in the target nucleic acid, or a complement thereof, and a sequence that encodes an RNA polymerase promoter; (b) synthesizing RNA using an RNA polymerase that recognizes the promoter; and (c) detecting the presence or absence of the resulting RNA using mass spectrometry thereby detecting the presence or absence of the target nucleic acid in the biological sample.
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