Method for In Vitro Assay of Demineralized Bone Matrix
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United States of America Patent
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app pub date -
Mar 8, 2007
filing date -
Dec 31, 2003
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Abstract
A sensitive, quantitative method to assess bone forming potential of demineralized bone matrix (DBM) is provided. Cortical DBM is treated with collagenase. The treated cortical DBM is placed in a cell culture for a dwell time. After the dwell time, the cell culture is observed for osteoblastic markers. The cortical bone so treated exhibits the same bone forming potential in vivo as untreated cortical bone.
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