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Abstract

Supported three-dimensional arrays of a polydiacetylene backbone bonded to and supported by a solid support in a sample are provided. The bonding is achieved by providing a support having thiol and/or amine groups and epoxy groups and/or maleimide groups, and a polydiacetylene backbone containing the other of thiol and/or amine groups or the epoxy and/or maleimide groups for reacting with the corresponding thiol groups or epoxy groups and/or maleimide groups on the support; provided that when an amine is used the other group is an epoxy; and/or having a layer of a polyelectrolyte on the support; and/or having a multiple atom linker between the three-dimensional array of the polydiacetylene backbone and the solid support. The supported three-dimensional arrays can be used for detecting an analyte; for evaluating the organic/water partition coefficient and/or oral absorptivity and/or ability of a compound to diffuse into cell membranes and/or transcellular permeability properties of a compound and/or the ionization state of a compound and/or the volume of distribution of a compound and/or the distribution of a compound into different tissues and/or the partitioning of a compound into cell organelles by monitoring the change in the fluorescence or phosphorescence upon exposure to the compound and optionally comparing it to a known change in fluorescence or phosphorescence, respectively. The supported three-dimensional arrays can also be used to evaluate the ability of a compound to bind to a protein and for the detection of a plurality of different species.

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