Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
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United States of America Patent
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Nov 24, 2009
Grant Date -
Nov 29, 2007
app pub date -
Mar 3, 2005
filing date -
Mar 3, 2004
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Abstract
This invention provides a method for determining the sequence of a DNA or an RNA, wherein (i) about 1000 or fewer copies of the DNA or RNA are bound to a solid substrate via 1,3-dipolar azide-alkyne cycloaddition chemistry and (ii) each copy of the DNA or RNA comprises a self-priming moiety.
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