OPTICAL APPARATUS FOR SIMULTANEOUSLY MEASURING THE SCATTERING AND CONCENTRATION OF SIGNALS OF MACROMOLECULES IN A FLOW CELL
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United States of America Patent
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Issued Date -
Dec 20, 2007
app pub date -
Apr 13, 2007
filing date -
Apr 13, 2006
priority date (Note) -
Abandoned
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Abstract
This invention relates to a fiber optic apparatus for simultaneously measuring the scattering and concentration signals of macromolecules in a flow cell 3. The apparatus is based on the delivery/focusing of both a laser and ultraviolet light source to the same physical position in a low volume flow cell 4, via a bifurcated optical fiber 3. This configuration allows the light scattering and concentration signal changes associated with a macromolecular solution passing through the flow channel to be measured simultaneously. This invention also relates to a method that uses the optical apparatus 10 to determine properties of a macromolecular solution such as the ideal crystallization and/or formulation conditions (via B.sub.22) for a given protein solution.
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