Method of Amplifying Nucleic Acid
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United States of America Patent
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Issued Date -
Dec 27, 2007
app pub date -
Dec 6, 2004
filing date -
Dec 10, 2003
priority date (Note) -
Abandoned
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Abstract
In the case where a template nucleic acid has sequences substantially the same as each other, a primer is designed in a region between these sequences so as to give a product in a ladder structure in which a target region is polymerized in a single sequence via the same sequences in the ICAN reaction. By using a chimeric oligonucleotide primer and a ladder-forming oligonucleotide primer containing a specific base sequence, an amplification product having a ladder structure can be positively formed and thus the sensitivity, amplification efficiency and reaction speed in the ICAN reaction can be elevated.
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