Importance

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Abstract

The binding reaction between an affinity substance as a measuring subject and a binding partner having binding affinity with the affinity substance is measured by agglutination reaction. The binding partner is linked to carrier particles, and the carrier particles agglutinate with each other by the binding reaction. The agglutination reaction is enhanced by incubating the reaction mixture before electric field application. Further, the agglutination reaction is enhanced by regulating the temperature or viscosity of reaction mixture placed in an electric field. These contribute to an increase of measuring sensitivity. Still further, a step of diluting the reaction mixture containing simple particles may be conducted prior to counting of agglutinates. This diluting step intensifies the binding between affinity substance and binding partner. As a result, any disintegration of agglutinates can be prevented, and the measuring sensitivity can be enhanced. Also, the carrier particles and agglutinates after the dilution can be discriminated from each other at high precision.

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First Claim