Genetic Screen for Interaction Interface Mapping
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United States of America Patent
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Issued Date -
Feb 21, 2008
app pub date -
May 31, 2004
filing date -
May 30, 2003
priority date (Note) -
Abandoned
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Abstract
The present invention provides improved reverse hybrid assay methods for identifying amino acid residues within a protein that are required for its interaction or physical association with another protein, wherein disruption of an interaction between a protein of interest and its binding partner protein is assayed for a library of mutations of said protein of interest, and maintenance of an interaction between the protein of interest and another binding partner is assayed simultaneously in a single step, thereby reducing the incidence of uninformative mutations in the protein of interest that are detected.
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