US Patent Application No: 2008/0057,505

Number of patents in Portfolio can not be more than 2000

METHODS AND COMPOSITIONS FOR DETECTING RARE CELLS FROM A BIOLOGICAL SAMPLE

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Abstract

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The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells. This invention further provides an efficient and rapid method to specifically remove red blood cells as well as white blood cells from a biological sample containing at least one of each of red blood cells and white blood cells, resulting in the enrichment of rare target cells including circulating tumor cells (CTC), stromal cells, mesenchymal cells, endothelial cells, fetal cells, stem cells, non-hematopoietic cells etc from a blood sample. The method is based upon combination of immuno-microparticles (antibody coated microparticles) and density-based separation. The final enriched target cells can be subjected to a variety of analysis and manipulations, such as flowcytometry, PCR, immunofluorescence, immunocytochemistry, image analysis, enzymatic assays, gene expression profiling analysis, efficacy tests of therapeutics, culturing of enriched rare cells, and therapeutic use of enriched rare cells. In addition, depleted plasma protein and white blood cells can be optionally recovered, and subjected to other analysis such as inflammation studies, gene expression profiling, etc.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
AVIVA BIOSCIENCES CORPORATIONSAN DIEGO, CA21

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cerny, David Crawfordsville, IN 12 261
Ghetti, Andrea San Diego, CA 6 20
Harvie, Gioulnar I San Diego, CA 4 20
Lin, Ping Belleville, CA 29 167
Shi, Wenge San Marcos, CA 4 20
Tang, Mengjia San Diego, CA 4 20
Tao, Guoliang San Diego, CA 20 145
Tao, Huimin San Diego, CA 6 65
Wu, Lei Sunnyvale, CA 111 1206
Xu, Jia Toronto, ON, CA 28 369

Patent Citation Ranking

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Patent Info (Count) # Cites Year
 
SMITH & NEPHEW, INC. (2)
8,273,253 Apparatus and method for filter cleaning by ultrasound, backwashing and filter movement during the filtration of biological samples 1 2008
8,777,017 Apparatus and method for filter cleaning by ultrasound, backwashing and filter movement during the filtration of biological samples 0 2012
 
AVIVA BIOSCIENCES CORPORATION (1)
8,569,009 Methods and compositions for detecting rare cells from a biological sample 0 2007
 
CELLSCAPE CORPORATION (1)
8,774,488 Method and device for identification of nucleated red blood cells from a maternal blood sample 0 2011
 
JEOL RESONANCE INC. (1)
7,463,032 NMR spectrometer and method of NMR measurement 1 2007
 
OZOLAB (1)
8,313,644 Bottle with an integrated filtration assembly that is manually operated using a plunger 2 2010
 
Other [Check patent profile for assignment information] (2)
7,767,087 Floating filter holder 5 2007
7,955,501 Floating filter holder 3 2010