US Patent Application No: 2008/0057,505

Number of patents in Portfolio can not be more than 2000

METHODS AND COMPOSITIONS FOR DETECTING RARE CELLS FROM A BIOLOGICAL SAMPLE

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Abstract

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The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells. This invention further provides an efficient and rapid method to specifically remove red blood cells as well as white blood cells from a biological sample containing at least one of each of red blood cells and white blood cells, resulting in the enrichment of rare target cells including circulating tumor cells (CTC), stromal cells, mesenchymal cells, endothelial cells, fetal cells, stem cells, non-hematopoietic cells etc from a blood sample. The method is based upon combination of immuno-microparticles (antibody coated microparticles) and density-based separation. The final enriched target cells can be subjected to a variety of analysis and manipulations, such as flowcytometry, PCR, immunofluorescence, immunocytochemistry, image analysis, enzymatic assays, gene expression profiling analysis, efficacy tests of therapeutics, culturing of enriched rare cells, and therapeutic use of enriched rare cells. In addition, depleted plasma protein and white blood cells can be optionally recovered, and subjected to other analysis such as inflammation studies, gene expression profiling, etc.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
AVIVA BIOSCIENCES CORPORATIONSAN DIEGO, CA25

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cerny, David San Diego, CA 9 310
Ghetti, Andrea San Diego, CA 8 46
Harvie, Gioulnar I San Diego, CA 3 42
Lin, Ping San Diego, CA 27 247
Shi, Wenge San Marcos, CA 3 42
Tang, Mengjia San Diego, CA 3 42
Tao, Guoliang San Diego, CA 18 349
Tao, Huimin San Diego, CA 6 113
Wu, Lei San Diego, CA 98 1949
Xu, Jia San Diego, CA 31 583

Cited Art Landscape

Patent Info (Count) # Cites Year
 
OY FARON PHARMACEUTICALS LTD. (1)
* 5,616,468 Compositions and diagnostic methods using monoclonal antibodies against CD44v6 14 1995
 
MCMASTER UNIVERSITY (1)
* 5,840,490 Telomerase activity associated with hematological and colorectal malignancies 10 1995
 
AVIVA BIOSCIENCES CORPORATION (2)
* 2003/0134,416 Methods, compositions, and automated systems for separating rare cells from fluid samples 33 2002
* 6,949,355 Methods, compositions, and automated systems for separating rare cells from fluid samples 49 2002
 
DENDREON PHARMACEUTICALS, INC. (1)
* 5,646,004 Methods for enriching fetal cells from maternal body fluids 64 1994
* Cited By Examiner

Patent Citation Ranking

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Patent Info (Count) # Cites Year
 
Other [Check patent profile for assignment information] (3)
* 7,767,087 Floating filter holder 9 2007
* 2008/0164,223 Floating filter holder 3 2007
7,955,501 Floating filter holder 8 2010
 
ONKOTEC GMBH (1)
* 2012/0190,057 Method for the qualitative and/or quantitative analysis of tumour cells 0 2010
 
UNIVERSITY OF MIAMI (1)
9,155,764 Expanded utility of red-cell derived microparticles (RMP) for treatment of bleeding 0 2012
 
GERON CORPORATION (1)
* 2012/0070,842 Assay for Telomerase Activity Using Microfluidic Device 1 2010
 
AVIVA BIOSCIENCES CORPORATION (8)
8,980,568 Methods and compositions for detecting non-hematopoietic cells from a blood sample 0 2005
* 2006/0252,054 Methods and compositions for detecting non-hematopoietic cells from a blood sample 38 2005
8,986,944 Methods and compositions for separating rare cells from fluid samples 0 2006
8,986,945 Methods and compositions for detecting rare cells from a biological sample 1 2007
8,569,009 Methods and compositions for detecting rare cells from a biological sample 0 2007
* 2008/0206,757 METHODS AND COMPOSITIONS FOR DETECTING RARE CELLS FROM A BIOLOGICAL SAMPLE 24 2007
8,969,021 Methods and compositions for detecting non-hematopoietic cells from a blood sample 0 2013
9,290,812 Methods and compositions for separating rare cells from fluid samples 0 2014
 
The General Hospital Corporation (2)
* 9,034,658 Microfluidic devices for the capture of biological sample components 0 2010
* 2013/0209,988 MICROFLUIDIC DEVICES FOR THE CAPTURE OF BIOLOGICAL SAMPLE COMPONENTS 2 2010
 
VERINATA HEALTH, INC (1)
* 2010/0304,978 Methods and compositions for identifying a fetal cell 23 2010
 
CELLSCAPE CORPORATION (1)
8,774,488 Method and device for identification of nucleated red blood cells from a maternal blood sample 5 2011
 
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM (1)
* 2012/0329,061 QUANTITATIVE RT-PCR DETECTION FOR GENES INVOLVED IN EPITHELIAL MESENCHYMAL TRANSITION IN PERIPHERAL BLOOD OF CANCER PATIENTS 0 2011
 
TUFTS UNIVERSITY (1)
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JEOL RESONANCE INC. (1)
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FLUIDIGM CORPORATION (1)
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SMITH & NEPHEW, INC. (3)
8,273,253 Apparatus and method for filter cleaning by ultrasound, backwashing and filter movement during the filtration of biological samples 7 2008
* 2010/0143,879 APPARATUS AND METHOD FOR FILTER CLEANING BY ULTRASOUND, BACKWASHING AND FILTER MOVEMENT DURING THE FILTRATION OF BIOLOGICAL SAMPLES 0 2008
8,777,017 Apparatus and method for filter cleaning by ultrasound, backwashing and filter movement during the filtration of biological samples 0 2012
 
OZOlab (2)
8,313,644 Bottle with an integrated filtration assembly that is manually operated using a plunger 18 2010
* 2011/0168,644 SYSTEMS AND METHODS FOR PERSONAL WATER FILTRATION 4 2010
 
WARSAW ORTHOPEDIC, INC. (1)
* 9,333,447 Filtration device 0 2012
 
UNIVERSITY OF SOUTHERN CALIFORNIA (2)
* 9,182,387 Methods for cancer detection, diagnosis and prognosis 0 2013
* 2014/0038,271 METHODS FOR CANCER DETECTION, DIAGNOSIS AND PROGNOSIS 1 2013
* Cited By Examiner