METHODS AND COMPOSITIONS FOR DETECTING RARE CELLS FROM A BIOLOGICAL SAMPLE

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United States of America Patent

PATENT NO 20080057505
APP PUB NO 20080057505A1
SERIAL NO

11777962

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells. This invention further provides an efficient and rapid method to specifically remove red blood cells as well as white blood cells from a biological sample containing at least one of each of red blood cells and white blood cells, resulting in the enrichment of rare target cells including circulating tumor cells (CTC), stromal cells, mesenchymal cells, endothelial cells, fetal cells, stem cells, non-hematopoietic cells etc from a blood sample. The method is based upon combination of immuno-microparticles (antibody coated microparticles) and density-based separation. The final enriched target cells can be subjected to a variety of analysis and manipulations, such as flowcytometry, PCR, immunofluorescence, immunocytochemistry, image analysis, enzymatic assays, gene expression profiling analysis, efficacy tests of therapeutics, culturing of enriched rare cells, and therapeutic use of enriched rare cells. In addition, depleted plasma protein and white blood cells can be optionally recovered, and subjected to other analysis such as inflammation studies, gene expression profiling, etc.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
AVIVA BIOSCIENCES CORPORATIONSAN DIEGO, CA26

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Cerny, David San Diego, CA 9 320
Ghetti, Andrea San Diego, CA 8 52
Harvie, Gioulnar I San Diego, CA 3 48
Lin, Ping San Diego, CA 29 269
Shi, Wenge San Marcos, CA 3 48
Tang, Mengjia San Diego, CA 3 48
Tao, Guoliang San Diego, CA 19 402
Tao, Huimin San Diego, CA 6 120
Wu, Lei San Diego, CA 100 2124
Xu, Jia San Diego, CA 36 644

Cited Art Landscape

Patent Info (Count) # Cites Year
 
OY FARON PHARMACEUTICALS LTD. (1)
* 5616468 Compositions and diagnostic methods using monoclonal antibodies against CD44v6 15 1995
 
MCMASTER UNIVERSITY (1)
* 5840490 Telomerase activity associated with hematological and colorectal malignancies 12 1995
 
AVIVA BIOSCIENCES CORPORATION (2)
* 2003/0134,416 Methods, compositions, and automated systems for separating rare cells from fluid samples 42 2002
* 6949355 Methods, compositions, and automated systems for separating rare cells from fluid samples 54 2002
 
DENDREON PHARMACEUTICALS, INC. (1)
* 5646004 Methods for enriching fetal cells from maternal body fluids 72 1994
* Cited By Examiner

Patent Citation Ranking

Forward Cite Landscape

Patent Info (Count) # Cites Year
 
Onkotec GmbH (1)
* 2012/0190,057 Method for the qualitative and/or quantitative analysis of tumour cells 0 2010
 
GERON CORPORATION (1)
* 2012/0070,842 Assay for Telomerase Activity Using Microfluidic Device 1 2010
 
UNIVERSITY OF MIAMI (1)
9155764 Expanded utility of red-cell derived microparticles (RMP) for treatment of bleeding 0 2012
 
AVIVA BIOSCIENCES CORPORATION (9)
8980568 Methods and compositions for detecting non-hematopoietic cells from a blood sample 2 2005
* 2006/0252,054 Methods and compositions for detecting non-hematopoietic cells from a blood sample 42 2005
8986944 Methods and compositions for separating rare cells from fluid samples 0 2006
8986945 Methods and compositions for detecting rare cells from a biological sample 1 2007
8569009 Methods and compositions for detecting rare cells from a biological sample 0 2007
* 2008/0206,757 METHODS AND COMPOSITIONS FOR DETECTING RARE CELLS FROM A BIOLOGICAL SAMPLE 27 2007
8969021 Methods and compositions for detecting non-hematopoietic cells from a blood sample 1 2013
9290812 Methods and compositions for separating rare cells from fluid samples 0 2014
9556485 Methods and compositions for detecting non-hematopoietic cells from a blood sample 0 2015
 
THE GENERAL HOSPITAL CORPORATION (2)
* 9034658 Microfluidic devices for the capture of biological sample components 0 2010
* 2013/0209,988 MICROFLUIDIC DEVICES FOR THE CAPTURE OF BIOLOGICAL SAMPLE COMPONENTS 2 2010
 
VERINATA HEALTH, INC. (1)
* 2010/0304,978 Methods and compositions for identifying a fetal cell 32 2010
 
CELLSCAPE CORPORATION (1)
8774488 Method and device for identification of nucleated red blood cells from a maternal blood sample 6 2011
 
BONVI WATER INC. (3)
* 7767087 Floating filter holder 9 2007
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HITACHI, LTD. (1)
9487747 Cell culture device 0 2012
 
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM (1)
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TUFTS UNIVERSITY (1)
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JEOL RESONANCE INC. (1)
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8273253 Apparatus and method for filter cleaning by ultrasound, backwashing and filter movement during the filtration of biological samples 12 2008
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8777017 Apparatus and method for filter cleaning by ultrasound, backwashing and filter movement during the filtration of biological samples 0 2012
 
CHANG GUNG UNIVERSITY (1)
* 2015/0233,932 Methods, Systems, and Compositions for Enrichment of Rare Cells 0 2014
 
OZOlab (2)
8313644 Bottle with an integrated filtration assembly that is manually operated using a plunger 21 2010
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WARSAW ORTHOPEDIC, INC. (1)
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BECTON, DICKINSON AND COMPANY (1)
9535065 Methods and compositions for cytometric detection of rare target cells in a sample 0 2012
 
UNIVERSITY OF SOUTHERN CALIFORNIA (2)
* 9182387 Methods for cancer detection, diagnosis and prognosis 0 2013
* 2014/0038,271 METHODS FOR CANCER DETECTION, DIAGNOSIS AND PROGNOSIS 1 2013
* Cited By Examiner