Importance

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Abstract

It is intended to provide a DNA amplification method whereby a single-stranded DNA in a desired direction and in a desired region can be conveniently and efficiently prepared. Namely, a DNA amplification method with the use of a DNA as a template which comprises: the first step wherein use is made of a first primer being complementary to the 3'-terminal side of a target base sequence, a second primer having a base sequence being homologous with the 5'-terminal side of the target base sequence, and a third primer having a base sequence in the 3'-terminal side being homologous with the second primer or a part of the 5'-terminal side base; sequence of the first primer, the melting temperature of the first primer Tm.sub.1, the melting temperature of the second primer Tm.sub.2 and the melting temperature of the third primer Tm.sub.3 have the relationships represented by the following formulae: (formulue) Tm.sub.1

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First Claim