Importance

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Abstract

A device for nucleic acid preparation includes an outer housing and an inner housing carried within the outer housing and rotatable with respect to the outer housing. The inner housing defines an inner chamber and includes inlet and outlet ports and a nucleic acid extraction substrate within the inner chamber. The outer housing includes a sample inlet port, wash fluid inlet and outlet ports, and elute fluid inlet and outlet ports, and the ports of the inner housing can be selectively aligned with ports of the outer housing by rotating the inner housing with respect to the outer housing. By aligning one or both ports of the inner housing with the appropriate ports of the outer housing, sample material can be introduced into the chamber and wash and elution fluids can be passed through the chamber. Nucleic acid from the sample introduced into the chamber is captured on the extraction substrate and is released from the extraction substrate by sonication prior to being eluted out of the chamber.

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First Claim