Method of Detecting Cystic Fibrosis Associated Mutations
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United States of America Patent
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Issued Date -
Jun 12, 2008
app pub date -
Jun 1, 2005
filing date -
Jun 1, 2004
priority date (Note) -
Abandoned
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Abstract
The present invention describes a method for the simultaneous identification of two or more single base changes, insertions, deletions or translocations in a plurality of target nucleotide sequences that are markers associated with cystic fibrosis. Multiplex detection is accomplished using multiplexed tagged allele specific primer extension (ASPE) and hybridization of such extended primers to a probe, preferably an addressable anti-tagged support.
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