BUFFER SOLUTION FOR ELECTROPORATION AND A METHOD COMPRISING THE USE OF THE SAME
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United States of America Patent
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app pub date -
Oct 31, 2007
filing date -
Apr 23, 2001
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Abandoned
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Abstract
A buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce the biologically active molecules into the cells using electric current. The buffer solution includes HEPES and at least 10 mmol*1.sup.-1 magnesium ions (Mg.sup.2+). The buffer solution has a buffer capacity of at least 20 mmol*1.sup.-1*pH.sup.-1 at a change in the pH from pH 7 to pH 8 and at a temperature of 25.degree. C. The buffer solution also has an ionic strength of at least 200 mmol*1.sup.-1.
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