Method for detecting a target using enzyme directed deposition of elemental metal
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United States of America Patent
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Feb 15, 2011
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app pub date -
Mar 4, 2008
filing date -
Mar 30, 2001
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Abstract
Disclosed embodiments concern site-specific, enzymatic-directed deposition of elemental metal for in-situ analysis. Enzyme substrates are contacted with metal ions and subsequently direct the deposition of elemental metals. Sensitive and selective detection of target molecules, such as biomarkers in various biological samples, can be obtained using various methods, such as in-situ chromogenic immunohistochemical (IHC) detection with bright field light microscopy.
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