In vitro techniques for use with stem cells
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United States of America Patent
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Apr 1, 2014
Grant Date -
Sep 11, 2008
app pub date -
Jun 1, 2005
filing date -
Jun 1, 2004
priority date (Note) -
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Abstract
A method is provided for use with extracted blood, including (a) applying blood to a first gradient suitable for selecting first-pass cells having a density less than 1.077 g/ml; (b) applying the first-pass cells to a second gradient suitable for selecting second-pass cells having a density between 1.055 and 1.074 g/ml; (c) increasing the number of cells having a density between 1.055 and 1.074 g/ml, by culturing the second-pass cells for a period lasting between 3 and 30 days; and (d) identifying endothelial progenitor cells in the cultured cells. Other embodiments are also described.
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