METHOD OF PURIFYING ACIDIC PROTEINS EXPRESSED IN PLANTS
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United States of America Patent
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Issued Date -
Nov 6, 2008
app pub date -
May 2, 2008
filing date -
May 2, 2007
priority date (Note) -
Abandoned
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Abstract
The present invention provides a rapid and relatively simple process for purification of acidic proteins expressed in tobacco cells. The process comprises three main purification steps: precipitation with polyethyleneimine, column chromatography with a hydrophobic interaction resin, and column chromatography with hydroxyapatite. The process provides pure or essentially pure protein at a very high yield.
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