Method of Generating Translationally Active Linear Dna Molecules and Use Thereof in Array Formats
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United States of America Patent
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Issued Date -
Dec 11, 2008
app pub date -
Feb 3, 2005
filing date -
Feb 3, 2005
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Abandoned
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Abstract
This invention provides a method for producing expressionally and translationally active linear DNA molecules that can be used as protein expression cassettes and are useful for high throughput protein expression and analysis in living cells. The method comprises a PCR amplification using two primers complementary to the sequences flanking the DNA sequence of interest, such as a cDNA, an open reading frame or a gene, that exists in expression vectors. The resultant PCR product contains a promoter, the DNA sequence of interest, and a termination sequence. The invention further provides different uses of these translationally active DNA cassettes, such as in proteome arrays.
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