Detection of polyketide synthetase gene expression in Karenia brevis
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United States of America Patent
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Feb 15, 2011
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Sep 8, 2008
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Oct 28, 2005
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Abstract
. Another aspect of the invention concerns NASBA primers and a molecular beacon that enable detection of mRNA of one of the PKS genes. In another aspect, the present invention includes an internal control RNA (IC-RNA) that enables quantitative detection of the target as well as correction for inhibition of amplification. The assay of the invention provides a rapid and specific detection of the putative toxin-producing genes of the Florida Red Tide Organism. The assay of the invention is useful for determining the virulence of red tide blooms.
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