METHODS AND COMPOSITIONS RELATING TO MULTIPLEXED GENOMIC GAIN AND LOSS ASSAYS
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United States of America Patent
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app pub date -
Nov 21, 2008
filing date -
Dec 23, 2005
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Abstract
Compositions and methods are provided for detecting genomic DNA gain and loss. Embodiments of inventive assays include using a substrate-attached composite nucleic acid probe which specifically hybridizes to two or more genomic loci in a genomic region of a reference genome. The genomic region is characterized by a first terminus and a second terminus and has an intermediate region disposed between the first terminus and second terminus of at least 400 kilobases. The composite nucleic acid probe includes nucleic acid sequences which specifically hybridize to substantially an entire first genomic locus including the first terminus and to substantially an entire second genomic locus including the second terminus. Methods and compositions are provided which include assessment of two or more genomic DNA references.
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