Methods for Flow Cytometry Analyses of Un-Lysed Cells from Biological Fluids
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United States of America Patent
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Issued Date -
Apr 23, 2009
app pub date -
May 11, 2007
filing date -
May 13, 2006
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Abstract
A method for analyzing a pathological deviation of at least one white blood cell population from a normal level in an un-lysed blood sample, comprising the steps of counting, with a flow cytometer, a number, n.sub.1, of white blood cells expressing a first marker; a number, n.sub.2, of white blood cells expressing a second marker, and a number, n.sub.3, of white blood cells expressing a third marker but not the first marker; and comparing the sum (n.sub.1+n.sub.2+n.sub.3) with a reference value. The sum (n.sub.1+n.sub.2+n.sub.3) may represent the number of lymphocytes. The first, second and third markers may be chosen from the group consisting of CD56, CD3 and CD19.
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