T7 phage peptide display system and uses thereof
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United States of America Patent
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Dec 22, 2015
Grant Date -
Apr 30, 2009
app pub date -
May 15, 2008
filing date -
May 15, 2007
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Abstract
A bacteriophage T7 display vector for expressing and displaying an exogenous peptide, wherein the display vector comprises a polynucleotide encoding a bacteriophage T7 tail fiber protein p17 in which a hepatocyte-targeting determinant sequence is inactivated, and wherein a cloning site is contained in a coding sequence for the tail fiber protein p17 or in a coding sequence for a capsid protein p10B. Also provided are a host cell containing the display vector as described above, a bacteriophage T7 particle comprising at least one copy of a p17 protein which comprises an exogenous peptide displayed thereon, or at least one copy of a p10 protein with an exogenous peptide displayed thereon, wherein a hepatocyte-targeting determinant sequence on the bacteriophage T7 tail fiber protein p17 is inactivated. The present invention further provides a viral lysate containing assembled bacteriophage T7 particle described above, and a method for determining if a candidate peptide is a liver-targeting peptide, or for screening for liver targeting peptides.
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- 15 United States
- 10 France
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- 5 Korea
- 2 Other
Patent Owner(s)
- WISCONSIN ALUMNI RESEARCH FOUNDATION
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Inventor(s)
Inventor Name | Address | # of filed Patents | Total Citations |
---|---|---|---|
Ludtke, James John | Madison, US | 1 | 5 |
Sokoloff, Alexander V | Madison, US | 5 | 49 |
Wolff, Jon Asher | Shorewood, US | 11 | 1011 |
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