Method for Gene Amplification
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United States of America Patent
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Issued Date -
Jun 11, 2009
app pub date -
Jul 18, 2006
filing date -
Nov 24, 2005
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The present invention provides a double-stranded DNA constructed specifically for high speed gene amplification, a method for gene amplification and a method for synthesizing protein. The gene amplification system of the present invention used a site-specific recombinase such as Cre-lox system and target sequence thereof to efficiently induce a type of replication referred to as a double rolling-circle replication (DRCR). Amplification unit, whose structure is shown in FIG. 2 (a), is constructed in animal and other cells. DRCR is induced by two recombination events triggered by a site-specific recombinase (Cre) when each replication folk progresses between each pair of target sequences (lox sequences).
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