METHOD FOR PRODUCTION OF CYTOCHROME P450 WITH N-TERMINAL TRUNCATED P450 REDUCTASE
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Nov 4, 2008
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May 5, 2006
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Abstract
The present invention provides a P450 reductase lacking N-terminal amino acids, as well as a nucleic acid encoding the P450 reductase. When co-expressed with a cytochrome P450, the P450 reductase increases the activity and/or expression of the cytochrome P450 compared to the activity and/or expression of the cytochrome P450 when co-expressed with a wild type P450 reductase. The invention also provides the use of certain promoters to increase expression of cytochrome P450, P450 reductase and/or b5, as well as the use of protease-deficient strains of yeast in which to express the proteins of the present invention.
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