Method of designing DNA probe chip for room temperature hybridization and the DNA probe chip
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United States of America Patent
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Oct 22, 2019
Grant Date -
Aug 13, 2009
app pub date -
Nov 7, 2008
filing date -
Nov 19, 2007
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Abstract
The present invention relates to a method of designing DNA probe chip for room-temperature hybridization in order to solve the solvent evaporation problem occurring when carrying out said hybridization at a high temperature of 40° C.˜50° C. or higher, wherein the method is designed to allow genotyping through hybridizing at a room temperature of 20° C.˜30° C. The method of designing DNA probe chip comprises designing DNA probe to start at −10˜+5 position that is between −10 position which is overlapped 10 sequences with primer and +5 position which is 5 sequences far from the 3′-terminal of primer, based on 0 position which is 3′-terminal of primer.
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