Devices and methods for detecting and quantitating nucleic acids using size-separation of amplicons
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United States of America Patent
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Issued Date -
Aug 27, 2009
app pub date -
Jan 22, 2009
filing date -
Jan 23, 2008
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Abstract
Devices and methods are described for detecting and quantifying nucleic acids using a sealed system that minimizes contamination. In particular, provided herein are devices for and methods using nucleic acid amplification that permit multiple sampling of an amplification reaction mixture and quantitation and identification of amplicons during the course of an amplification reaction. Methods involving the transfer of samples from an amplification reaction mixture into a separation network, separation of nucleic acids based on size, and identification and quantitation of nucleic acids are disclosed.
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