Method For Enrichment Involving Elimination By Mismatch Hybridisation
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United States of America Patent
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Issued Date -
Sep 24, 2009
app pub date -
Feb 17, 2005
filing date -
Feb 17, 2004
priority date (Note) -
Abandoned
status (Latency Note)
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Abstract
The present invention relates to a method for selecting a molecule from a library of such molecules associated with identifier oligonucleotides, said molecule having affinity towards a target. The method involves contacting library with a target to allow for an interaction between the molecules and the target and partitioning a fraction enriched in identifier oligonucleotides of molecules interacting with the target. After an optional nucleic acid amplification of the partitioned fraction, the fraction is subjected to conditions at which hetero-duplexes and homo-duplexes are formed. The homo-duplexes are subsequently recovered and decoded to identify the identity of the molecule interacting with the target.
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