MASS SPECTROMETRY METHODS FOR MULTIPLEXED QUANTIFICATION OF PROTEIN KINASES AND PHOSPHATASES
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United States of America Patent
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Issued Date -
Oct 8, 2009
app pub date -
May 4, 2007
filing date -
May 5, 2006
priority date (Note) -
Abandoned
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Abstract
The inventions relates to methods and kits for capture and/or analysis of kinases and/or phosphatases in one or more samples. In some embodiments, a kinase inhibitor, e.g. staurosporine or its derivative, is used to capture kinases from a sample. In some embodiments, a phosphatase inhibitor, e.g. microcystin or its derivative, is used to capture phosphatases from a sample. Methods for quantitative analysis of captured kinases and/or proteases are also provided. In some embodiments, quantitative analysis is accomplished using mass spectrometry. In addition, the invention provides kits related to same.
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