METHOD OF SEQUENCING AND MAPPING TARGET NUCLEIC ACIDS
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United States of America Patent
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Issued Date -
Oct 29, 2009
app pub date -
Apr 24, 2009
filing date -
Apr 24, 2008
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Abandoned
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Abstract
The present teachings pertain to methods, compositions, reaction mixtures, and kits for mapping a low complexity sequence to a locus in a genome. In some embodiments, the low complexity sequence can be used to determine the methylation profile of a target nucleic acid. A strand-replacing reaction results in a product containing a first strand and a second strand, which can be connected together with a stem-loop adapter to form a single strand. A sequencing reaction can compare the two strands of the product, allowing the experimentalist to both map the sequence to a locus in a reference genome, as well as ascertain the methylation profile of the original target nucleic acid.
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