METHOD FOR PRODUCTION OF PLANT CELL HAVING CHROMOSOME LOSS
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United States of America Patent
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Issued Date -
Dec 24, 2009
app pub date -
Dec 8, 2005
filing date -
Dec 8, 2004
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Abandoned
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Abstract
The present invention provides a simple aneuploid production process, applicable to all species, without producing unexpected damage to chromosomes other than a chromosome which is to be disappeared. A vector comprising two site-specific recognition sequences oriented in the opposite direction, or one site-specific recombinase recognition sequence comprising a point-symmetric nucleotide sequence, and a recombinase gene is introduced into a plant cells or a vector comprising two site-specific recognition sequences oriented in the opposite directions or one site-specific recombinase recognition sequence comprising a point-symmetric nucleotide sequence is introduced into a plant cell; a recombinase is allowed to act transiently in the cell during at least growth of the cell; and the cell is cultured and grown; and a cell in which a predetermined chromosome is disappeared is selected.
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