Method for Dissociation of Cells
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United States of America Patent
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Issued Date -
Jan 14, 2010
app pub date -
Jul 8, 2009
filing date -
Jul 9, 2008
priority date (Note) -
Abandoned
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Abstract
Disclosed is a method for the dissociation of cells. Cells are processed under conditions of pH, temperature, and shear to thereby yield a mixture of cell wall ghosts and cytoplasm. Preferably, the cells are jet cooked at an alkaline pH to form an intermediate mixture, and the intermediate mixture is subsequently subjected to mechanical homogenization. Generally, the cells become dissociated, whereby at least one separate cell wall component is substantially separate from the dissociated cell walls.
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